Flexible foraging behaviour increases predator vulnerability to climate change.

Higher temperatures are expected to reduce species coexistence by increasing energetic demands. However, flexible foraging behaviour could balance this effect by allowing predators to target specific prey species to maximize their energy intake, according to principles of optimal foraging theory. Here we test these assumptions using a large dataset comprising 2,487 stomach contents from six fish species with different feeding strategies, sampled across environments with varying prey availability over 12 years in Kiel Bay (Baltic Sea). Our results show that foraging shifts from trait- to density-dependent prey selectivity in warmer and more productive environments. This behavioural change leads to lower consumption efficiency at higher temperature as fish select more abundant but less energetically rewarding prey, thereby undermining species persistence and biodiversity. By integrating this behaviour into dynamic food web models, our study reveals that flexible foraging leads to lower species coexistence and biodiversity in communities under global warming.

Quantitative description of six fish species' gut contents and prey abundances in the Baltic Sea (1968-1978).

The dataset presents a compilation of stomach contents from six demersal fish species from two functional groups inhabiting the Baltic Sea. It includes detailed information on prey identities, body masses, and biomasses recovered from both the fish's digestive systems and their surrounding environment. Environmental parameters, such as salinity and temperature levels, have been integrated to enrich this dataset. The juxtaposition of information on prey found in stomachs and in the environment provides an opportunity to quantify trophic interactions across different environmental contexts and investigate how fish foraging behaviour adapts to changes in their environment, such as an increase in temperature. The compilation of body mass and taxonomic information for all species allows approaching these new questions using either a taxonomic (based on species identity) or functional trait (based on body mass) approach.

A framework for untangling the consequences of artificial light at night on species interactions.

Although much evidence exists showing organismal consequences from artificial light at night (ALAN), large knowledge gaps remain regarding ALAN affecting species interactions. Species interactions occur via shared spatio-temporal niches among species, which may be determined by natural light levels. We review how ALAN is altering these spatio-temporal niches through expanding twilight or full Moon conditions and constricting nocturnal conditions as well as creating patches of bright and dark. We review literature from a database to determine if ALAN is affecting species interactions via spatio-temporal dynamics. The literature indicates a growing interest in ALAN and species interactions: 58% of the studies we analysed have been published since 2020. Seventy-five of 79 studies found ALAN altered species interactions. Enhancements and reductions of species interactions were equally documented. Many studies revealed ALAN affecting species interactions spatially, but few revealed temporal alterations. There are biases regarding species interactions and ALAN-most studies investigated predator-prey interactions with vertebrates as predators and invertebrates as prey. Following this literature review, we suggest avenues, such as remote sensing and animal tracking, that can guide future research on the consequences of ALAN on species interactions across spatial and temporal axes. This article is part of the theme issue 'Light pollution in complex ecological systems'.

Facing the infinity: tackling large samples of challenging Chironomidae (Diptera) with an integrative approach.

Background: Integrative taxonomy is becoming ever more significant in biodiversity research as scientists are tackling increasingly taxonomically challenging groups. Implementing a combined approach not only guarantees more accurate species identification, but also helps overcome limitations that each method presents when applied on its own. In this study, we present one application of integrative taxonomy for the highly abundant and particularly diverse fly taxon Chironomidae (Diptera). Although non-biting midges are key organisms in merolimnic systems, they are often cast aside in ecological surveys because they are very challenging to identify and extremely abundant. Methods: Here, we demonstrate one way of applying integrative methods to tackle this highly diverse taxon. We present a three-level subsampling method to drastically reduce the workload of bulk sample processing, then apply morphological and molecular identification methods in parallel to evaluate species diversity and to examine inconsistencies across methods. Results: Our results suggest that using our subsampling approach, identifying less than 10% of a sample's contents can reliably detect >90% of its diversity. However, despite reducing the processing workload drastically, the performance of our taxonomist was affected by mistakes, caused by large amounts of material. We conducted misidentifications for 9% of vouchers, which may not have been recovered had we not applied a second identification method. On the other hand, we were able to provide species information in cases where molecular methods could not, which was the case for 14% of vouchers. Therefore, we conclude that when wanting to implement non-biting midges into ecological frameworks, it is imperative to use an integrative approach.

A global agenda for advancing freshwater biodiversity research.

Global freshwater biodiversity is declining dramatically, and meeting the challenges of this crisis requires bold goals and the mobilisation of substantial resources. While the reasons are varied, investments in both research and conservation of freshwater biodiversity lag far behind those in the terrestrial and marine realms. Inspired by a global consultation, we identify 15 pressing priority needs, grouped into five research areas, in an effort to support informed stewardship of freshwater biodiversity. The proposed agenda aims to advance freshwater biodiversity research globally as a critical step in improving coordinated actions towards its sustainable management and conservation.

Towards Insect-Friendly Road Lighting-A Transdisciplinary Multi-Stakeholder Approach Involving Citizen Scientists.

(1) The project "Tatort Streetlight" implements an insect-friendly road light design in a four year before-after, control-impact (BACI) approach involving citizen scientists. It will broaden the stakeholder interests from solely anthropogenic perspectives to include the welfare of insects and ecosystems. Motivated by the detrimental impacts of road lighting systems on insects, the project aims to find solutions to reduce the insect attraction and habitat fragmentation resulting from roadway illumination. (2) The citizen science approach invites stakeholders to take part and join forces for the development of a sustainable and environmentally friendly road lighting solution. Here, we describe the project strategy, stakeholder participation and motivation, and how the effects of the alternative road luminaire and lighting design can be evaluated. (3) The study compares the changes in (a) insect behavior, (b) night sky brightness, and (c) stakeholder participation and awareness. For this purpose, different experimental areas and stakeholders in four communities in Germany are identified. (4) The project transfers knowledge of adverse effects of improperly managed road illumination and interacts with various stakeholders to develop a new road lighting system that will consider the well-being of street users, local residents, and insects.

Expanding conservation culturomics and iEcology from terrestrial to aquatic realms.

The ongoing digital revolution in the age of big data is opening new research opportunities. Culturomics and iEcology, two emerging research areas based on the analysis of online data resources, can provide novel scientific insights and inform conservation and management efforts. To date, culturomics and iEcology have been applied primarily in the terrestrial realm. Here, we advocate for expanding such applications to the aquatic realm by providing a brief overview of these new approaches and outlining key areas in which culturomics and iEcology are likely to have the highest impact, including the management of protected areas; fisheries; flagship species identification; detection and distribution of threatened, rare, and alien species; assessment of ecosystem status and anthropogenic impacts; and social impact assessment. When deployed in the right context with awareness of potential biases, culturomics and iEcology are ripe for rapid development as low-cost research approaches based on data available from digital sources, with increasingly diverse applications for aquatic ecosystems.

Societal attention toward extinction threats: a comparison between climate change and biological invasions.

Public attention and interest in the fate of endangered species is a crucial prerequisite for effective conservation programs. Societal awareness and values will largely determine whether conservation initiatives receive necessary support and lead to adequate policy change. Using text data mining, we assessed general public attention in France, Germany and the United Kingdom toward climate change and biological invasions in relation to endangered amphibian, reptile, bird and mammal species. Our analysis revealed that public attention patterns differed among species groups and countries but was globally higher for climate change than for biological invasions. Both threats received better recognition in threatened than in non-threatened species, as well as in native species than in species from other countries and regions. We conclude that more efficient communication regarding the threat from biological invasions should be developed, and that conservation practitioners should take advantage of the existing attention toward climate change.

iEcology: Harnessing Large Online Resources to Generate Ecological Insights.

Digital data are accumulating at unprecedented rates. These contain a lot of information about the natural world, some of which can be used to answer key ecological questions. Here, we introduce iEcology (i.e., internet ecology), an emerging research approach that uses diverse online data sources and methods to generate insights about species distribution over space and time, interactions and dynamics of organisms and their environment, and anthropogenic impacts. We review iEcology data sources and methods, and provide examples of potential research applications. We also outline approaches to reduce potential biases and improve reliability and applicability. As technologies and expertise improve, and costs diminish, iEcology will become an increasingly important means to gain novel insights into the natural world.

The global decline of freshwater megafauna.

Freshwater ecosystems are among the most diverse and dynamic ecosystems on Earth. At the same time, they are among the most threatened ecosystems but remain underrepresented in biodiversity research and conservation efforts. The rate of decline of vertebrate populations is much higher in freshwaters than in terrestrial or marine realms. Freshwater megafauna (i.e., freshwater animals that can reach a body mass ≥30 kg) are intrinsically prone to extinction due to their large body size, complex habitat requirements and slow life-history strategies such as long life span and late maturity. However, population trends and distribution changes of freshwater megafauna, at continental or global scales, remain unclear. In the present study, we compiled population data of 126 freshwater megafauna species globally from the Living Planet Database and available literature, and distribution data of 44 species inhabiting Europe and the United States from literature and databases of the International Union for Conservation of Nature and NatureServe. We quantified changes in population abundance and distribution range of freshwater megafauna species. Globally, freshwater megafauna populations declined by 88% from 1970 to 2012, with the highest declines in the Indomalaya and Palearctic realms (-99% and -97%, respectively). Among taxonomic groups, mega-fishes exhibited the greatest global decline (-94%). In addition, freshwater megafauna experienced major range contractions. For example, distribution ranges of 42% of all freshwater megafauna species in Europe contracted by more than 40% of historical areas. We highlight the various sources of uncertainty in tracking changes in populations and distributions of freshwater megafauna, such as the lack of monitoring data and taxonomic and spatial biases. The detected trends emphasize the critical plight of freshwater megafauna globally and highlight the broader need for concerted, targeted and timely conservation of freshwater biodiversity.

Susceptibility of European freshwater fish to climate change: Species profiling based on life-history and environmental characteristics.

Climate change is expected to strongly affect freshwater fish communities. Combined with other anthropogenic drivers, the impacts may alter species spatio-temporal distributions and contribute to population declines and local extinctions. To provide timely management and conservation of fishes, it is relevant to identify species that will be most impacted by climate change and those that will be resilient. Species traits are considered a promising source of information on characteristics that influence resilience to various environmental conditions and impacts. To this end, we collated life-history traits and climatic niches of 443 European freshwater fish species and compared those identified as susceptible to climate change to those that are considered to be resilient. Significant differences were observed between the two groups in their distribution, life history, and climatic niche, with climate-change-susceptible species being distributed within the Mediterranean region, and being characterized by greater threat levels, lesser commercial relevance, lower vulnerability to fishing, smaller body and range size, and warmer thermal envelopes. Based on our results, we establish a list of species of highest priority for further research and monitoring regarding climate-change susceptibility within Europe. The presented approach represents a promising tool to efficiently assess large groups of species regarding their susceptibility to climate change and other threats, and to identify research and management priorities.

On the occurrence of three non-native cichlid species including the first record of a feral population of Pelmatolapia (Tilapia) mariae (Boulenger, 1899) in Europe.

Thermally influenced freshwater systems provide suitable conditions for non-native species of tropical and subtropical origin to survive and form proliferating populations beyond their native ranges. In Germany, non-native convict cichlids (Amatitlania nigrofasciata) and tilapia (Oreochromis sp.) have established populations in the Gillbach, a small stream that receives warm water discharge from a local power plant. Here, we report on the discovery of spotted tilapia (Pelmatolapia mariae) in the Gillbach, the first record of a reproducing population of this species in Europe. It has been hypothesized that Oreochromis sp. in the Gillbach are descendants of aquaculture escapees and our mtDNA analysis found both O. mossambicus and O. niloticus maternal lineages, which are commonly used for hybrids in aquaculture. Convict cichlids and spotted tilapia were most probably introduced into the Gillbach by aquarium hobbyists. Despite their high invasiveness worldwide, we argue that all three cichlid species are unlikely to spread and persist permanently beyond the thermally influenced range of the Gillbach river system. However, convict cichlids from the Gillbach are known to host both native and non-native fish parasites and thus, non-native cichlids may constitute threats to the native fish fauna. We therefore strongly recommend continuous monitoring of the Gillbach and similar systems.

Predicting the consequences of species loss using size-structured biodiversity approaches.

Understanding the consequences of species loss in complex ecological communities is one of the great challenges in current biodiversity research. For a long time, this topic has been addressed by traditional biodiversity experiments. Most of these approaches treat species as trait-free, taxonomic units characterizing communities only by species number without accounting for species traits. However, extinctions do not occur at random as there is a clear correlation between extinction risk and species traits. In this review, we assume that large species will be most threatened by extinction and use novel allometric and size-spectrum concepts that include body mass as a primary species trait at the levels of populations and individuals, respectively, to re-assess three classic debates on the relationships between biodiversity and (i) food-web structural complexity, (ii) community dynamic stability, and (iii) ecosystem functioning. Contrasting current expectations, size-structured approaches suggest that the loss of large species, that typically exploit most resource species, may lead to future food webs that are less interwoven and more structured by chains of interactions and compartments. The disruption of natural body-mass distributions maintaining food-web stability may trigger avalanches of secondary extinctions and strong trophic cascades with expected knock-on effects on the functionality of the ecosystems. Therefore, we argue that it is crucial to take into account body size as a species trait when analysing the consequences of biodiversity loss for natural ecosystems. Applying size-structured approaches provides an integrative ecological concept that enables a better understanding of each species' unique role across communities and the causes and consequences of biodiversity loss.

Individual variation in functional response parameters is explained by body size but not by behavioural types in a poeciliid fish.

Functional responses are per-capita feeding rate models whose parameters often scale with individual body size but the parameters may also be further influenced by behavioural traits consistently differing among individuals, i.e. behavioural types or animal personalities. Behavioural types may intrinsically lead to lower feeding rates when consistently shy, inactive and easily stressed individuals cannot identify or respond to risk-free environments or need less food due to lower metabolic rates linked to behaviour. To test how much variation in functional response parameters is explained by body size and how much by behavioural types, we estimated attack rate and handling time individually for differently sized female least killifish (Heterandria formosa) and repeatedly measured behavioural traits for each individual. We found that individual fish varied substantially in their attack rate and in their handling time. Behavioural traits were stable over time and varied consistently among individuals along two distinct personality axes. The individual variation in functional responses was explained solely by body size, and contrary to our expectations, not additionally by the existing behavioural types in exploration activity and coping style. While behavioural trait-dependent functional responses may offer a route to the understanding of the food web level consequences of behavioural types, our study is so far only the second one on this topic. Importantly, our results indicate in contrast to that previous study that behavioural types do not per se affect individual functional responses assessed in the absence of external biotic stressors.

Body size and the behavioral ecology of insects: linking individuals to ecological communities.

The role of body size as a key feature determining the biology and ecology of individual animals, and thus the structure and dynamics of populations, communities, and ecosystems, has long been acknowledged. Body size provides a functional link between individual-level processes such as physiology and behavior, with higher-level ecological processes such as the strength and outcome of trophic interactions, which regulate the flow of energy and nutrients within and across ecosystems. Early ecological work on size in animals focused on vertebrates, and especially mammals. More recent focus on invertebrates, and insects in particular, that spans levels of organization from individual physiology to communities, has greatly expanded and improved our understanding of the role of body size in ecology. Progress has come from theoretical advances, from the production of new, high-resolution empirical data sets, and from enhanced computation and analytical techniques. Recent findings suggest that many of the allometric concepts and principles developed over the last century also apply to insects. But these recent studies also emphasize that while body size plays a crucial role in insect ecology, it is not the entire story, and a fuller understanding must come from an approach that integrates both size and non-size effects. In this review we discuss the core principles of a size-based (allometric) approach in insect ecology, together with the potential of such an approach to connect biological processes and mechanisms across levels of organization from individuals to ecosystems. We identify knowledge gaps, particularly related to size constraints on insect movement and behavior, which can impact the strength and outcome of species interactions (and especially trophic interactions) and thus link individual organisms to communities and ecosystems. Addressing these gaps should facilitate a fuller understanding of insect ecology, with important basic and applied benefits.

Bringing animal personality research into the food web arena.

While the concept of consistent behavioural differences among individuals of the same population has gained a lot of scientific attention over the last decade, its implementation into a community context with a focus on species-level interactions is still in its infancy. In their study on the effects of animal personalities on predator-prey functional responses of mussel-eating crabs, Toscano & Griffen (2014) introduce a promising avenue for future research synthesizing concepts and ideas from animal behaviour and food web ecology. More precisely, by showing that the interplay of animal personalities and predator and prey body sizes significantly alters the outcome of predator-prey interactions, this study provides important evidence that the concept of animal personalities needs greater consideration if we want to refine and improve current models of predator-prey interactions and the impact of individual-level variation on quantitative food-web dynamics.

Body masses, functional responses and predator-prey stability.

The stability of ecological communities depends strongly on quantitative characteristics of population interactions (type-II vs. type-III functional responses) and the distribution of body masses across species. Until now, these two aspects have almost exclusively been treated separately leaving a substantial gap in our general understanding of food webs. We analysed a large data set of arthropod feeding rates and found that all functional-response parameters depend on the body masses of predator and prey. Thus, we propose generalised functional responses which predict gradual shifts from type-II predation of small predators on equally sized prey to type-III functional-responses of large predators on small prey. Models including these generalised functional responses predict population dynamics and persistence only depending on predator and prey body masses, and we show that these predictions are strongly supported by empirical data on forest soil food webs. These results help unravelling systematic relationships between quantitative population interactions and large-scale community patterns.